Oakland University William Beaumont School of Medicine Publication List

Many attempts have been made to improve wound healing using growth factors and a large array of medications. This study reexamined the effect of Botox on second degree burn wound in rats. Histological alterations and temporal evolution of two cytokines TGFβ1, and TNF-α were assessed in presence and absence of Botox. Fifty adult Sprague Dawley rats were used; one group was injected 10 units of Botox into wound. Daily observation and transepidermal water loss were assessed. Punch biopsies were taken on days 0, 3, 8, 14 and 28 for RNA extraction and PCR, and for routine light microscopy. Botox treated rats showed a delayed inflammation, necrosis, and earlier crust detachment compared to controls. On day 14, a better and smoother epithelialization was noted in the Botox groups. Finally, by day 28, the wound surface area was smaller and the quality of the skin was better with more fibroblasts in the regenerated dermis of Botox group, which expressed 2-4 times more TGFβ1. It peaked on days 3 and 8 and then decreased to almost half the control group by day 28. On the other hand, the TNF-α peaked immediately after burn to almost 20 times the control, then decreased between 2.72 to 5.5 times during the rest of the experiment very much like TGFβ1, or lower. Botox could possibly play an alleviating role and have a positive effect on burn wound healing. Jurjus AR, Hajj Hussein I and Eid A (2012). "Health tourism quality in Lebanon: the first world health service at a third world cost." 16th International Conference on ISO & TQM & 8th Cooperation & Competition Conference, Linnaeus University, Vaxjo, Sweden: 1. Full-Text Department of Biomedical Sciences Jurjus AR, Hajj Hussein I and Gerges AA (2012). Knowledge, attitude, behavior, and practice of the UNIFIL peacekeepers on human immunodeficiency virus, (ed). Sexually Transmitted Infections: INTECH: 219. Full-Text Department of Biomedical Sciences Jury RP, Thibodeau BJ, Fortier LE, Geddes TJ, Ahmed S, Pruetz BL, Farinola MA and Wilson GD (2012). "Gene expression changes associated with the progression of intraductal papillary mucinous neoplasms." Pancreas 41(4): 611-618. Full-Text Department of Surgery Department of Radiation Oncology OBJECTIVES: The diagnosis of high-grade intraductal papillary mucinous neoplasm (IPMN) is difficult to distinguish from low-grade IPMN. The aim of this study was to identify potential markers for the discrimination of high-grade and invasive (HgInv) IPMN from lowand moderate-grade dysplasia IPMN. METHODS: Laser capture microdissection was used to isolate distinct foci of low-grade, moderate-grade, high-grade, and invasive IPMN from paraffin-embedded archival tissue from 14 patients who underwent resection for IPMN. Most samples included multiple grades in the same specimen. Affymetrix Human Exon microarrays were used to compare lowand moderate-grade dysplasia IPMN with HgInv IPMN. RESULTS: Sixty-two genes were identified as showing significant changes in expression (P </= 0.05 and a 2-fold cutoff), including up-regulation of 41 in HgInv IPMN. Changes in gene expression are associated with biological processes related to malignant behavior including cell motion, cell proliferation, response to hypoxia, and epithelial-to-mesenchymal transition. In addition, altered signaling in several transforming growth factor beta-related pathways was exhibited in the progression of IPMN to malignancy. CONCLUSIONS: This study identifies a set of genes associated with the progression of IPMN to malignancy. These genes are potential markers that could be used to identify IPMN requiring surgical resection.